Fig. 7 | Scientific Reports

Fig. 7

From: Anti-fibrogenic effect of umbilical cord–derived mesenchymal stem cell–conditioned media in human esophageal fibroblasts

Fig. 7

UC-MSC-CM decreases the TGF-β1-induced phosphorylation of Smad2. HEFs were treated with TGF-β1 (5 ng/mL) and cultured with or without UC-MSC-CM for 48 h. (A) Representative western blots showing the protein expression of phosphorylated Smad2 (p-Smad2) and total Smad2 with GAPDH as a loading control. (B) Quantitation of p-Smad2 using western blot analyses (n = 4). Data are expressed as the mean ± SEM. #P < 0.05 versus the control; *P < 0.05 versus TGF-β1 treatment only (ANOVA w/ Tukey). Original images of blots are presented in Supplementary Fig. S5.

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