Fig. 4 | Scientific Reports

Fig. 4

From: Structure-function analysis of time-resolved immunological phases in metabolic dysfunction-associated fatty liver disease (MASH) comparing the NIF mouse model to human MASH

Fig. 4

Temporal dynamics of Global liver transcriptomics. (a) RNA-seq strategy overview: Libraries prepared from total liver RNA of NIF mice and 2,4αβ.NOD.Rag2+/− control mice (n = 3/3, n = 3/3, n = 2/2 mice, for 3 weeks, 6 weeks and 18 weeks, respectively) at indicated timepoints. (b) Principal component analysis (PCA), each data point represents an individual mouse. (c-h). Volcano plots of differentially expressed genes (DEGs) in 2,4αβ.NOD.Rag2+/− control (c-e) and NIF mice (f-h) comparing 3 vs. 6 weeks (c, f), 3 vs. 18 weeks (d, g), and 6 vs. 18 weeks (e, h). DEGs were identified using DESeq2 default Wald’s test and adjusted by the Benjamini-Hochberg (BH) method. Dashed lines indicate an adjusted p-value < 0.05 and logFC > 1. (i-k) Heatmaps show top 25 up- and down-regulated genes in NIF livers at different ages, adjusted p-value cutoff at p < 0.05. Each cell represents relative DEG values of normalized variance stabilized transformed gene expression, with samples in columns and genes in rows.

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