Fig. 2 | Scientific Reports

Fig. 2

From: Aligning fermentation conditions with non-canonical amino acid addition strategy is essential for Nε-((2-azidoethoxy)carbonyl)-L-lysine uptake and incorporation into the target protein

Fig. 2

Bench-scale bioreactor cultivations of E. coli BL21(DE3) strains producing wt FTN2 or SHC133Z. (A,B) run #1; (C,D) run #2; (E,F) run #3. (A,C,E) Calculated CDM (grey lines) and calculated Azk concentrations (blue lines), measured CDM for wt FTN2 (black triangles) and SHC133Z (blue circles), black arrows indicate induction times. (B,D,F) Fab production yield quantified via ELISA (n = 3 technical replicates, SDM).

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