Fig. 1 | Scientific Reports

Fig. 1

From: Value creation of copra meal mannan into functional manno-oligosaccharides (β-MOS) using the mannanase Bacillus man B (BlMan26B)

Fig. 1

Optimization of bioconversion reaction and analysis of β-MOS products. (a) TLC analysis of products from various reaction conditions of substrate and enzyme at various time points (0, 1, 2, 3, 6, 9, 12, 16, 20, and 24 h). (b) HPAEC-PAD analysis of MOS composition at optimal reaction conditions. Galactomanno-oligosaccharides and manno-oligosaccharides standards were used for comparison and product quantitation. The corresponding retention times observed were 11.67 min for mannobiose, 14.65 min for mannotriose, 19.35 min for mannotretraose, and 36.42 min for mannohexaose. O-GMM3, 61-α-D-Galactosyl-mannotriose; O-GM3, 61-α-D-Galactosyl-mannobiose plus mannotriose.

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