Fig. 6 | Scientific Reports

Fig. 6

From: Value creation of copra meal mannan into functional manno-oligosaccharides (β-MOS) using the mannanase Bacillus man B (BlMan26B)

Fig. 6

A summary of this research. The bioconversion of defatted copra meal from virgin coconut oil manufacturing into functional manno-oligosaccharides (β-MOS) by enzymatic hydrolysis using β-mannanase from Bacillus licheniformis (BlManB) was investigated in this study. The size of oligosaccharides, analyzed by TLC and HPLC, obtained ranged from dimers to hexamers with 36.7% conversion yields. The in vitro growth promoting effects of β-MOS were demonstrated on various probiotic bacteria compared to other oligosaccharides. In vitro toxicity assays of β-MOS on human dermal fibroblasts and monocytes showed no cytotoxic effect. For the first time, β-MOS at a concentration ranging from 10 to 200 µg/mL also demonstrated potent anti-inflammatory activity against LPS-induced inflammation of human macrophage THP-1, in a dose-dependent manner.

Back to article page