Fig. 7
5/TM11 effect on cytokines expression and release in murine macrophages. (A, B) Expression of cytokines in treated RAW264.7 cultures. Cells were co-treated for 6 h with LPS (1 µg/mL) and 2/TM7n, 5/TM11 (10 µM) or DMSO as control. Expression levels were assessed via qRT-PCR, using RPLP0 as housekeeping gene. Data were normalized to LPS + DMSO condition. Data analysed and plotted with GraphPad Prism as mean ± s.d. of at least three independent experiments. (C) Secretion of cxcl10 in RAW264.7. Cxcl10 secretion was measured via ELISA on RAW264.7 supernatant. Cells were co-treated for 6 h with LPS (1 µg/mL) and 2/TM7n, 5/TM11(10 µM) or DMSO as control. The relative quantity of Cxcl10 pg/mL for each sample was normalized according to the number of cells quantified through Crystal Violet assay. Data were normalized to LPS + DMSO as control, and numbers are expressed as a percentage. Data analysed and plotted with GraphPad Prism as mean ± s.d. of three independent experiments. (D, E) Expression of cytokines in treated RAW264.7 cultures. Cells were stimulated for 2 h with LPS (1 µg/mL) and subsequently treated with 2/TM7n, 5/TM11 (10 µM) or DMSO as control for 4 h. Expression levels were assessed via qRT-PCR, using RPLP0 as a housekeeping gene. Data were normalized to LPS + DMSO condition. Data analysed and plotted with GraphPad Prism as mean ± s.d. of at least three independent experiments. (F) Secretion of cxcl10 in RAW264.7 cells. Cxcl10 secretion was measured via ELISA on RAW264.7 supernatant. Cells were stimulated for 2 h with LPS (1 µg/mL) and subsequently treated with 2/TM7n, 5/TM11 (10 µM) or DMSO. The relative quantity of Cxcl10 pg/mL for each sample was normalized according to the number of cells quantified through Crystal Violet assay. Data were normalized to LPS + DMSO as control, and numbers are expressed as a percentage. Data analysed and plotted with GraphPad Prism as mean ± s.d. of three independent experiments.
