Fig. 9
From: Persistence of post-stress blood pressure elevation requires activation of astrocytes
Double fluorescent immunostaining of c-Fos (red) and GFAP (green) with DAPI (blue) staining in the rostral ventral medulla (RVM). (a) Condition a without arundic acid pre-treatment and without stress loading; (b) Condition b: without arundic acid pre-treatment and with stress loading; (c) Condition c: with arundic acid pre-treatment and without stress loading; (d) Condition d: with arundic acid pre-treatment and with stress loading. GFAP positive fibers were densely distributed just beneath the ventral medullary surface and constituted the glia limitans (marginal glial layer). Under Condition b, a number of c-Fos positive cells were observed in the superficial ventral medullary region, i.e., in the cardiovascular region RVM, Although the cells with arrows appeared yellow or orange, they were not double-positive for c-Fos and GFAP; their c-Fos-positive nuclei and GFAP-positive structures were not precisely aligned on the same z-axis planes. Bottom oblique line, ventral medullary surface. The anatomical portion of the depicted brain region is shown in Fig. S3. Scale bar; 100 μm.
