Fig. 2 | Scientific Reports

Fig. 2

From: Bio-printing method as a novel approach to obtain a fibrin scaffold settled by limbal epithelial cells for corneal regeneration

Fig. 2

Characteristics of the bioprinting process. Cell density and viability of cells extruded from the 3D printer nozzle at selected time points for 60 min (A). Stability of the expected density of extruded cells during the bioprinting process at 1, 2 and 3 min of extrusion (B). Imaging of calcein (green)/PI (red) cell staining for manually seeded (C, D) and bioprinted (E, F) samples on the day of culture initiation and after 7 days of culture. The green color indicates the high cell viability. The red color indicates the dead cells. Graph of cell viability seeded manually and bioprinted at two time points (G). Bioprinted cells were significantly more viable than manually deposited control on the day of culture initiation (*p < 0.001, n = 39) and also after 7 days of culture (*p < 0.001, n = 58). Scalebar = 1 mm.

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