Fig. 3
MiR-148b-3p inhibits cell proliferation, migration, and invasion. (a) Effects of miR-148b-3p on cell proliferation in human brain endothelial cells (HBECs). The cell growth rates were determined for 5 days using a WST-1 assay in untreated HBECs (HBECs group), HBECs treated with miRNA-negative control mimic (miR-NC mi group), and HBECs treated with miRNA-148b-3p mimic (miR-148b-3p mi group). (b) Representative microscopic images of cell migration and invasion by miR-148b-3p in HBECs using a wound healing assay and a trans-well assay, respectively. (c) Percentage of the wound closure area at 24 h. (d) Quantification of the number of invasive cells per field. Error bars indicate mean ± S.D. of three independent experiments. **P < 0.01, ***P < 0.001 when compared with untreated HBECs or HBECs treated with miR-NC. miR-NC mi miRNA-negative control mimic, miR-148b-3p mi miRNA-148b-3p mimic.
