Fig. 9

Cytotoxicity and apoptosis of simultaneously seeded mono and co-cultured spheroids from primary patient cells. Primary ovarian cancer cells and human peritoneal fibroblasts of the same patient were seeded into ULA plate and grown for 96 h as described above. Spheroids were treated after 72 h for another 24 h with 100 µM cisplatin or PBS. (A, B) Cell toxicity of UF-403-co-culture spheroids was measured by fluorescence microscopy using CellTox Green 24 h after treatment. (A) The fluorescence signals after treatment were quantified (relative fluorescence units RFU). Quantitative data are means ± SEM, N = 3, one-way ANOVA, ** (p < 0.01), **** (p < 0.0001). (B) Representative microscopic images of monocellular and multicellular spheroids after CellTox Green staining. Scale bar 500 μm. (C) Viability and caspase activity were measured after 24 h treatment (relative luminescence units RLU). Quantitative data are means ± SEM, N = 3, one-way ANOVA. (D) Viable cells were stained with calcein-AM in green, while death cells were marked in red with propidium iodide. Scale bars, 200 μm.