Fig. 3

Differential responses of NKG2D^High and NKG2D^Low CD8 T cells cocultured with PANC-1 cells treated withMICB siRNA. (a) MICB mRNA expression of PANC-1 cells treated with control (−) or MICB (+) siRNA. Data are mean with SEM from three independent experiments. (b) IFNG mRNA expression of NKG2D^High (left) and NKG2D^Low (right) CD8 T cells cocultured with PANC-1 cells treated with control (−) or MICB (+) siRNA. Data are mean with SEM from three independent experiments. (c) Expression of membrane MICB on PANC-1 cells treated with control (−) or MICB (+) siRNA. Data are mean with SEM from three independent experiments. (d) Concentration of soluble MICB in culture supernatant of PANC-1 cells treated with control (−) or MICB (+) siRNA. Data are mean with SEM from three independent experiments. (e) Cytotoxicity of NKG2D^High (open) and NKG2D^Low (closed) CD8 T cells against PANC-1 cells treated with control (−) or MICB (+) siRNA. Data are expressed as ratio of PANC-1/T cell number and are mean with SEM from three independent experiments. ns, not significant.