Fig. 5

Enzymatic activity of tag-free hIDS produced in N. benthamiana. Purified tag-free hIDS were incubated with an artificial substrate p-nitrocatechol sulfate dipotassium salt (4-PNCS) at 37^oC for 24 h. Blank, assay buffer alone; hIDS/HEH293, commercial hIDS produced in HEK293 used as positive control; hIDS, hIDS produced without hSUMF1; hIDS-su(1:1 and 1:4), hIDS produced with co-expression of hSUMF1 at ratios of 1:1 and 1:4, respectively. Error bars indicate the mean ± standard deviation (n = 5). Statistical data analysis was performed by Student’s t test (GraphPad Prism 6) and asterisks indicate significant differences (***p < 0.0001).