Fig. 6
From: Production of active human iduronate-2-sulfatase (IDS) enzyme in Nicotiana benthamiana
The effect of pHs and temperatures on the enzymatic activity of plant-produced hIDS-su. (A) Effect of pHs on the activity of hIDS-su. Purified hIDS-su was incubated at different pH values for 24 h of reaction before measuring enzymatic activity. The preincubated samples were used for enzymatic activity assay at pH 5.0 ± 0.2. (B) Effect of temperature on the activity of hIDS-su. Purified hIDS-su was incubated at various temperatures such as 4oC, 25oC, 37oC, 45oC, and 60oC for 24 h before reaction and the preincubated proteins were used for enzymatic activity measurement at 37oC. The activities at the standard conditions (pH 5.0 and 37oC) at time 24 h were taken as 100%. The activities at various pH values and temperatures were reported as relative activity to those at the standard conditions. The data were presented as mean ± standard deviation (n = 5).
