Fig. 4
From: The senolytic drug ABT-263 accelerates ovarian aging in older female mice
Effects of ABT-263 treatment on cell proliferation and apoptosis in aged mice. (A) Representative fluorescence images of ovarian sections stained with anti-PCNA antibody. Green rectangle enclosed region were magnified in the insets and indicates MGCs. (B) Statistical analysis of the percentage of PCNA positive cells per follicle. n = 3 in each group. (C) Representative images of ovarian sections stained with anti-γ-H2AX antibody. The boxed regions were magnified in the insets. The red triangle indicates γ-H2AX positive cells. (D) Statistical analysis of the number of γ-H2AX positive cells in the ovarian stroma. n = 3 in theY group, n = 6 in the O group, and n = 4 in the A group. (E) Representative images of ovarian sections stained with anti-caspase 3 antibody. The boxed regions were magnified in the insets. (F) Statistical analysis of the number of caspase 3 positive cells in the ovarian stroma. n = 3 in the Y group, n = 6 in the O group, and n = 4 in the A group. MGCs: multinucleated giant cells; CL: corpus luteum; GF: graafian follicle; SC: stromal cell. Y: young group; O: old group; A: old group treated with ABT-263. Compared with the Y group: *P < 0.05.
