Fig. 4
From: Comparison of human pluripotent stem cell differentiation protocols to generate neuroblastoma tumors
Protocol #4 generates tumors with the highest level of PHOX2B. (A) Schematic of generating tumors using each differentiation protocol in TRE-FLAG-MYCN transduced iPSC. (B) Western blot analysis of EDi27 and EDi28 TRE-FLAG-MYCN iPSC untreated and treated with doxycycline (100 ng/mL) for 24 h. (C) Kaplan Meier survival curves of mice implanted with (left) EDi27 (n = 4 per group) and (right) EDi28 (n = 4 per group) TRE-MYCN SA cells differentiated using each of the four protocols. (D) Western blot for expression of FLAG-MYCN in two tumors for each protocol compared to EDi27 iPSC. (E) Histology analysis of EDi27 MYCN tumors derived from each protocol showing (top) H&E staining and (bottom) IHC for PHOX2B. Scale bars = 100 um.
