Fig. 1
Schematic representation of the experimental system. Multiple accessions of seeds of five legume species stored at room temperature (Ambient) or -18 °C (Cold) for more than ten years were ordered in different biological replicates (R) based on their size. The replicates were analyzed with the LIANA© light analyzer and subsequently used for germination test, following the ISTA rules for each species. At the end of the germination test, several indices (G%, Z, MGT, root and shoot length) were calculated and the germination percentage data was integrated into the extracted CSV file containing the UPE and DL data for the same replicates. The complete datasets were independently used for the development of machine learning models for germination prediction using RapidMiner® software.
