Fig. 1

Experimental design and pre-requisites for assessing postprandial changes in healthy individuals. Detailed demographic information of the healthy participants (A). Schematic representation of the experimental setup, wherein blood samples were collected from healthy participants before and 75 min after consumption of an isocaloric meal. Samples were then processed to obtain plasma and plasma EVs, of which the later were used for detailed characterization by proteomics and flow cytometry (B). Bar chart illustrating the triacylglycerides levels in plasma before and after food intake, indicating postprandial changes (C). Bar plots displaying total protein concentrations in plasma and plasma-EVs, both pre- and postprandially (D). Correlation plot demonstrating no correlation between fasting time and total protein concentration in EV samples both in pre- and postprandial time points (E). n = 12, all samples were measured in technical triplicates. Statistical analyses were performed using paired t-test (C–D) and Pearson correlation (E), respectively. P values: * for p ≤ 0.05; ** for p ≤ 0.001; *** for p ≤ 0.0001.