Fig. 2
From: Nerve injury inhibits Oprd1 and Cnr1 transcription through REST in primary sensory neurons
SNI leads to transcriptomic activation of Rest and repression of opioid receptor and cannabinoid CB1 receptor genes in the injured DRG of wild-type mice. mRNA samples from the L3/L4 DRG tissues of 3 individual male mice were examined by RNA-Seq analyses. (a) Hierarchical clustering of differentially expressed genes between SNI and Sham surgery groups. (b) Volcano plot showing differentially expressed genes. (c) Differential expression analysis of Rest, Oprd1, Oprk1, Oprl1, Oprm1, and Cnr1 between SNI and Sham surgery groups. The size of the circle is -log10(FDR). (d) Gene Set Enrichment Analysis shows downregulation of the opioid signaling pathway. Experiments were performed in triplicate. NES represents Normalized Enrichment Score.
