Fig. 2
From: Deciphering the intracellular forces shaping mitochondrial motion
Mitochondrial motion regimes depend on cytoskeleton integrity. (a) Representative confocal image of a X. laevis melanophore cell expressing EGFP-XTP (green: microtubules) and incubated with MitoTracker Deep Red FM (red: mitochondria). Time-lapse images of a mitochondrion (white square, left panel) and its respective track are shown in the right panel and Supplementary Video S1. (b) Mitochondrial motion regimes. Tracking of three mitochondria experiencing sub-diffusive (orange, \(\alpha <1\), Supplementary Video S2), diffusive (dark red, \(\alpha \sim 1\), Supplementary Video S3), and super-diffusive (purple, \(\alpha >1\), Supplementary Video S4) behavior (top panel). The trajectories of the CM corresponding to these organelles and the MSD dependence with \(\tau\) for each of them are shown at the bottom panel. (c) MSD curves obtained for mitochondria within melanocytes in control condition (CTRL, N=89). (d) Distribution of \(\alpha\) values registered for mitochondria in control cells (CTRL: green), cells treated with nocodazole (NOC: blue, partial depolymerization of microtubules, N=49) or latrunculin-B (LAT: orange, depolymerization of F-actin, N=48) and cells transfected with mCherry-vim(1-138) (\(\hbox {VIM}^-\): red, disruption of vimentin filaments, N=46). (e) Generalized diffusion coefficient (\(D^*\)) distribution obtained for each experimental condition. The asterisks denote significant differences (p-value < 0.05) with respect to CTRL.
