Table 4 Experimental design RSM based on CCD with actual and predicted response for EPS production.
Std. | Run | Point type | Carbon source g L−1 | Nitrogen source g L−1 | pH | Bacterial concentration ml L−1 | EPS g L−1 | |
|---|---|---|---|---|---|---|---|---|
Actual | Predict | |||||||
26 | 1 | Center | 30 | 30 | 8 | 30 | 3.1 | 3.15 |
5 | 2 | Fact | 20 | 20 | 9 | 20 | 1.67 | 1.89 |
29 | 3 | Center | 30 | 30 | 8 | 30 | 3.28 | 3.15 |
27 | 4 | Center | 30 | 30 | 8 | 30 | 3.26 | 3.15 |
22 | 5 | Axial | 30 | 30 | 10 | 30 | 0 | 0.2 |
9 | 6 | Fact | 20 | 20 | 7 | 40 | 6.5 | 6.3 |
24 | 7 | Axial | 30 | 30 | 8 | 50 | 3.64 | 3.54 |
28 | 8 | Center | 30 | 30 | 8 | 30 | 3 | 3.15 |
15 | 9 | Fact | 20 | 40 | 9 | 40 | 1.12 | 1.04 |
30 | 10 | Center | 30 | 30 | 8 | 30 | 3.19 | 3.15 |
3 | 11 | Fact | 20 | 40 | 7 | 20 | 3.42 | 3.52 |
19 | 12 | Axial | 30 | 10 | 8 | 30 | 3.19 | 3.09 |
23 | 13 | Axial | 30 | 30 | 8 | 10 | 3.24 | 3.16 |
21 | 14 | Axial | 30 | 30 | 6 | 30 | 6.83 | 6.85 |
1 | 15 | Fact | 20 | 20 | 7 | 20 | 6.5 | 6.45 |
18 | 16 | Axial | 50 | 30 | 8 | 30 | 2.81 | 2.72 |
17 | 17 | Axial | 10 | 30 | 8 | 30 | 3.67 | 3.58 |
14 | 18 | Fact | 40 | 20 | 9 | 40 | 0.81 | 0.77 |
11 | 19 | Fact | 20 | 40 | 7 | 40 | 3.6 | 3.82 |
20 | 20 | Axial | 30 | 50 | 8 | 30 | 1.1 | 1.04 |
25 | 21 | Center | 30 | 30 | 8 | 30 | 3.09 | 3.15 |
7 | 22 | Fact | 20 | 40 | 9 | 20 | 1 | 0.91 |
16 | 23 | Fact | 40 | 40 | 9 | 40 | 1.5 | 1.67 |
10 | 24 | Fact | 40 | 20 | 7 | 40 | 5 | 5.21 |
4 | 25 | Fact | 40 | 40 | 7 | 20 | 3.6 | 3.46 |
8 | 26 | Fact | 40 | 40 | 9 | 20 | 0.86 | 1.13 |
6 | 27 | Fact | 40 | 20 | 9 | 20 | 0.79 | 0.68 |
2 | 28 | Fact | 40 | 20 | 7 | 20 | 4.8 | 4.95 |
13 | 29 | Fact | 20 | 20 | 9 | 40 | 1.32 | 1.57 |
12 | 30 | Fact | 40 | 40 | 7 | 40 | 4.32 | 4.17 |
