Table 1 Specific sequences of probes and primers (5′–3′).

From: A novel methylation-detection panel for HPV associated high-grade squamous intraepithelial lesion and cervical cancer screening

Gene

FAM19A4

EPB41L3

PAX1

ACTB

Forward primer

GGGTCGGTTTTTTTTTCGTT

TTTTTTGTTGTAGGAGATATCGAGG

GTAGTGACGGGAATTAATGAGTTG

TGGTGATGGAGGAGGTTTAGTAAGT

Reverse primer

GAACCCTAAATCCGTCTTCCTT

CCCTACGCCAAAACGAAAA

CAAACCCAAAATAAACTTCATCC

AACCAATAAAACCTACTCCTCCCTTAA

Probe

TAGGCGTTTTTTTCGAGCGTTTCG

CGGGGGTTTACGTTTAGAGAT

ATTGTCGAGATTGACGTGGAGGATACG

ACCACCACCCAACACACAATAACA

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