Fig. 5
JAML promoted CRC cell proliferation by activating the PI3K-AKT-mTOR signalling pathway in vivo. (A–C) LOVO cells transfected with the JAML knockdown virus or control cells were subcutaneously inoculated into BALB/c nude mice, after which tumours were subcutaneously implanted after culture. The tumour volumes of the mice were recorded regularly. The two groups of mice were sacrificed on the 40th day, after which the tumour tissue was weighed and embedded. (D) The tumour growth curve results showed that the subcutaneous implanted tumours in the LOVOshJAML group grew significantly more slowly than those in the control group. (E–K) Immunohistochemical staining results showing that in tumour tissues with low JAML expression, the expression of P-PI3KTyr467/199, P-AKTSer473 and P-mTORSer2448Ser2448 decreased.
