Fig. 4 | Scientific Reports

Fig. 4

From: Dynamic reciprocal interactions between activated T cells and tumor associated macrophages drive macrophage reprogramming and proinflammatory T cell migration within prostate tumor models

Fig. 4

T cells affect MDM behavior. (A) Primary M0 MDMs were cultured either in mono-culture (M0 Mono) for 10 days; or cultured step-wise as mono-culture, to co-culture with established LNCaP or 22Rv1 tumor cells, followed by a 24 h tri-culture with autologous T cells at either 250 or 5000 cells per well (Tri-LN 250, Tri-22 250, Tri-LN 5 K, Tri-22 5 K). The M0 Mono culture condition served as shared, matched internal control for both tumor cell co-culture configurations, represented in both data sets. (B) mRNA expression in MDMs in mono-culture, and all four tri-culture conditions expressed as normalized relative quantity (NRQ), as related to house-keeping genes RPLP0 and POLR2A. One-way ANOVA nonparametric with Friedman post-hoc analysis comparing the mean rank of each column with the rank of every other column; n = 5; error bar is mean with SEM. (C,D) Cytokine and chemokine protein concentrations (pg/mL) in cell cultures supernatant captured by multi-analyte bead assay. One-way ANOVA nonparametric with Friedman post-hoc analysis comparing the mean rank of each column with the rank of every other column; n = 5; Data represent mean ± SEM.

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