Fig. 1 | Scientific Reports

Fig. 1

From: Profiling of urinary extracellular vesicle protein signatures from patients with cribriform and intraductal prostate carcinoma in a cross-sectional study

Fig. 1The alternative text for this image may have been generated using AI.

Urinary EV characterization by western blot and NTA. (A) CD63 and Alix western blot of representative urinary EV samples from each major clinical groups. (B) Non-EV markers GRP75 (endoplasmic reticulum marker) and TOM20 (mitochondrial marker) as markers of transmembrane, lipid-bound and soluble proteins associated to other intracellular compartments than plasma membrane and endosomes. The process of transforming the original blot image into the final annotated image is outlined at the end of the supplementary documentation. NTA particle size distribution analysis of a representative urinary EV sample from (C) BPD and (D) IDC/Crib (insert represents a zoom in). Particle size mode distribution (E) and number of particles per protein amount (F) across major clinical subgroups. The central line inside each box represents the median value. Whiskers extend from the edges of the box to the minimum and maximum values within 1.5 times the interquartile range (IQR). Individual data points beyond the whiskers are considered outliers and are plotted individually. “c” indicates a mitochondrial enriched fraction from the large diffuse B cell lymphoma cell line HT.

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