Fig. 1
From: ETFDH mutation involves excessive apoptosis and neurite outgrowth defect via Bcl2 pathway
The apoptosis assays. (A: wild type and B: Mutant) Flow cytometry showed the increased apoptosis in the mutant-type cells. In the diagram, the fourth quadrant (Q4) represents healthy living cells (FITC-/PI-), the third quadrant (Q3) represents early apoptotic cells (FITC+/PI-), the second quadrant (Q2) represents late apoptotic cells (FITC+/PI+), and the first quadrant (Q1) represents dead cells. (C) the cell apoptosis index of wild type and mutant cells. Apoptosis index (%) = early apoptosis rate (Q3) + late apoptosis rate (Q2). (n = 2). (D) The TUNEL stain was performed to detect the apoptotic cells. The scale bar is 50 μm in length. (E) The TUNEL positive cell counts of the wild type cells and the mutant cells. n = 11 ~ 14. The statistical significance of the different protein expressions was estimated by two-tailed student’s t-test analysis. (*: p < 0.05, **: p < 0.01, ***: p < 0.001).
