Fig. 2
In vitro characterization of the SETBP1 antibody PA5-96609 for zebra finches. (a) A cropped western blot analysis of protein lysates of HEK cells transfected, from left to right, with pMAX, empty vector, zebra finch SETBP1-isoA and zebra finch SETBP1-isoB and detected with the SETBP1 antibody. A specific band with the expected size was only seen in the lysates that expressed zebra finch SETBP1. Original western blot is presented in Supplementary Fig. 1. (b) Immunodetection of SETBP1 in HEK cells transfected with zebra finch SETBP1-isoA (i-ii) and SETBP1-isoB (iii-iv) or with an empty vector (v-vi) and no primary antibody controls (NPC ii, iv and vi). Scale bar = 20 μm.
