Fig. 3 | Scientific Reports

Fig. 3

From: Expression and regulation of SETBP1 in the song system of male zebra finches (Taeniopygia guttata) during singing

Fig. 3

In vivo characterization of the SETBP1 antibody for zebra finches. The strong staining (a) was abolished or strongly reduced on brain slices when the SETBP1 antibody was pre-incubated with HEK protein lysates overexpressing SETBP1-Iso A (b) or SETBP1-Iso B (c). This was not the case when lysate of cells transfected with an empty vector was used for pre-incubation (d). No staining was observed in no primary antibody control (e). All photos were taken in mesopallium, a region with a high expression of zebra finch SETBP1 (supplementary Fig. 2). Scale bar = 20 μm.

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