Fig. 6
SETBP1 co-localizes with FoxP1 and PV in Area X, HVC and RA. (a) SETBP1 (color-coded in red) and FoxP1 (pseudo color-coded in yellow) immunostainings showed colocalization in HVC, RA and Area X. SETBP1 is expressed in projection neurons in HVC and medium spiny neurons in Area X. (b) SETBP1 (color-coded in red) and Parvalbumin (pseudo color-coded in yellow) immunostainings showing colocalization in HVC, RA and Area X. SETBP1 is also expressed in interneurons in HVC and Area X. In all areas we show DAPI (color-coded in blue) and merge of all channels. Orthogonal views of the co-localizing cells are show in each panel. Arrows indicate examples of a neuron co-expressing SETBP1 with either FoxP1 or PV. Scale bar = 20 μm. (c) Box plots showing the mean percentage of zebra finch SETBP1 and FoxP1 co-localizing neurons in Area X under different conditions (US, DS, NS and juvenile). (d) Box plots showing the mean percentage of zebra finch SETBP1 and PV co-localizing neurons in Area X in different conditions (US, DS, NS and juvenile). Sample size (number of birds): mean of US = 7, DS = 6, NS = 5 and Juveniles = 5. No statistically significant differences between the experimental conditions were detected, one-way ANOVA, Tukey’s Multiple Comparison Test (values in supplementary Table 2).
