Fig. 4 | Scientific Reports

Fig. 4

From: Evaluation of inter- and intra-variability in gut health markers in healthy adults using an optimised faecal sampling and processing method

Fig. 4

Comparison of variation of faecal pre-processing methods within replicate of the same pooled faecal sample. (A) Replicates (n = 10) of a pooled faecal sample, of which 5 were pre-processed by hammering-only, and 5 by both hammering and mill-homogenising. (B) The coefficient of variation percentage as calculated within the replicates of hammered-only samples (circles) and within replicates of mill-homogenised samples (triangles). (C,D) The untargeted metabolomics PCA scores plot based on features detected in positive ionization mode (C) and in negative ionization mode (D) of the hammered-only (circles) and mill-homogenised replicates (triangles).

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