Table 4 Comparison of faecal pre-processing methods within replicates from a pooled sample that were either hammered-only or mill-homogenised.
Hammered-only (n = 5 replicates) | Mill-homogenised (n = 5 replicates) | Pre-processing comparison | |||
|---|---|---|---|---|---|
Coefficient of variation % between replicates1 | Mean ± SD | Coefficient of variation % between replicates1 | Mean ± SD | P-value2 | |
pH | 0.43 | 7.46 ± 0.03 | 0.38 | 7.45 ± 0.03 | 0.63 |
Water content % | 1.63 | 71.3 ± 1.16 | 1.46 | 71.4 ± 1.04 | 1.00 |
Acetic acid (µmol/mg) | 23.1 | 3.79 ± 0.88 | 8.29 | 4.18 ± 0.35 | 0.44 |
Propionic acid (µmol/mg) | 19.0 | 1.36 ± 0.26 | 6.79 | 1.53 ± 0.1 | 0.19 |
Butyric acid µmol/mg | 17.6 | 1.59 ± 0.28 | 7.95 | 1.81 ± 0.14 | 0.19 |
Valeric acid (µmol/mg) | 15.0 | 0.25 ± 0.04 | 8.20 | 0.28 ± 0.02 | 0.13 |
Heptanoic acid (µmol/mg) | 14.5 | 0.01 ± 0 | 24.3 | 0.01 ± 0 | 0.81 |
Hexanoic acid (µmol/mg) | 14.8 | 0.12 ± 0.02 | 9.80 | 0.14 ± 0.01 | 0.06 |
Isobutyric acid (µmol/mg) | 16.7 | 0.19 ± 0.03 | 7.05 | 0.21 ± 0.01 | 0.19 |
Isovaleric acid (µmol/mg) | 15.9 | 0.3 ± 0.05 | 8.25 | 0.34 ± 0.03 | 0.13 |
4-methyl valeric acid (µmol/mg) | 6.54 | 0.01 ± 0 | 12.5 | 0.01 ± 0 | 0.19 |
Total SCFAs (µmol/mg) | 20.4 | 7.12 ± 1.45 | 7.53 | 7.96 ± 0.60 | 0.19 |
Total BCFAs (µmol/mg) | 15.9 | 0.5 ± 0.08 | 7.82 | 0.56 ± 0.04 | 0.19 |
Calprotectin (µg/g) | 15.5 | 53.5 ± 8.27 | 6.34 | 48.2 ± 3.06 | 0.19 |
Myeloperoxidase (ng/g) | 24.2 | 243 ± 58.9 | 48.7 | 273 ± 133 | 1.00 |
All metabolites positive ionization mode | 17.8 ± 19.7 | Not quantified | 16.6 ± 19.8 | Not quantified | N.A. |
All metabolites negative ionization mode | 16.1 ± 18.7 | Not quantified | 12.9 ± 13.3 | Not quantified | N.A. |
