Fig. 2
Quantitative mRNA expression by Fluidigm real-time RT-PCR for the NANOG gene (A, B) and POU5F1, NANOG, and C-MYC in ESCs (C). A Quantitative mRNA expression by Fluidigm real-time RT-PCR for the NANOG gene in different passages of undifferentiated SSCs. Quantitative mRNA analysis of the NANOG gene has shown that by increasing the passage in undifferentiated SSCs, the mRNA level of NANOG also increases and finally has a significant expression in passage 15 compared to passages 1 and 2. B Quantitative mRNA expression by Fluidigm real-time RT-PCR showed that NANOG has High mRNA expression levels in mouse embryonic stem cells and ES-like cells. In contrast, it has lower expression in Epiblast cells, and in P1 undifferentiated SSCs, NANOG has a significantly higher mRNA expression level than P9 undifferentiated SSCs. C Quantitative mRNA expression by Fluidigm real-time RT-PCR for the NANOG gene in different passages of differentiated SSCs. mRNA expression analysis of the NANOG gene showed significant expression in P4 and P5 of differentiated SSCs in contrast with P0 and P1, although they do not differ significantly from each other. D Quantitative mRNA expression by Fluidigm real-time RT-PCR for NANOG, POU5F1, and C-MYC showed significantly higher expression of NANOG than POU5F1 and C-MYC, respectively, in mouse embryonic stem cells. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
