Fig. 4
From: Proteomic analysis of CD29+ Müller cells reveals metabolic reprogramming in rabbit myopia model
Validation of CD29+ cell identity as Müller cells. (a) Comparison of CD29+ and CD29- cells revealed that CD29+ cells exhibited larger and more elongated morphology, characteristic of Müller cells, as opposed to CD29- cells. The cytoplasm of CD29+ cells showed a significantly stronger CD29 immunofluorescence signal, indicating effective isolation. (b) Quantitative analysis showed a significant increase in mean CD29 immunofluorescence intensity in the retina of myopic rabbits compared to controls. (c) Immunofluorescence staining of CD29+ cells from both the central and peripheral retinal regions demonstrated co-expression of glial fibrillary acidic protein (GFAP) and glutamine synthetase (GLUL), confirming their identity as Müller cells.
