Fig. 3 | Scientific Reports

Fig. 3

From: An Andrias davidianus derived composite hydrogel with enhanced antibacterial and bone repair properties for osteomyelitis treatment

Fig. 3

Investigation of the antibacterial and osteogenic properties of the SOA hydrogel in vitro. A General schematic illustration of antibacterial and osteogenic studies. B Cytocompatibility of the SSAD-based osteomyelitis alleviator (SOA) detected using the MTT assay. SSAD: SSAD hydrogel; +HA: SSAD/HA hydrogel; +Van: SSAD/HA + Van hydrogel; +PNC: SSAD/HA + Van + PVAm/antagomir-NC hydrogel; +P214: SSAD/HA + Van + PVAm/antagomir-214 hydrogel; +PMNC: SSAD/HA + Van + PVAm/MSA hydrogel; SOA: SSAD/HA + Van + PVAm/MSA-anti214 hydrogel. C Images of the spread plate method. D Validation of the long-term antibacterial activity of the + Van hydrogel. E Quantification of the results in (C). F Quantification of the results in (D). G Representative images of alkaline phosphatase (ALP) staining and alizarin red staining (ARS) of human mesenchymal stem cells (hMSCs) after treatment with each hydrogel component. HAlp and Runx2 expression levels in hMSCs treated with each hydrogel component as detected by real-time polymerase chain reaction. All quantitative data are presented as mean values ± standard deviation (n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, ns: no significance).

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