Fig. 7

Histological staining of bone repair in osteomyelitis mice. A Immunohistochemical images of osteocalcin (OCN) in bone sections of each group. The black scale bar indicates 500 μm, and the red scale bar in the black box indicates 50 μm. B Quantification of relative integrated optical density (IOD) values of OCN immunostaining using Image-Pro Plus 6.0 software (https://en.freedownloadmanager.org/users-choice/Image-pro_Plus6.0_Free_Download.html). C Masson’s trichrome staining images of bone sections from each group (the newly formed bone is marked with a black arrow). The black scale bar indicates 500 μm, and the red scale bar in the black box indicates 50 μm. D Goldner’s trichrome staining images of bone sections from each group (newly formed bone is marked with a red arrow). The black scale bar indicates 500 μm, and the red scale bar in the black box indicates 50 μm. All quantitative data are presented as mean values ± standard deviation (n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, ns: no significance).