Fig. 1

Morphological analysis of Leptopira interrogans KeTo. Light (a), fluorescence (b) and transmission electron microscopic images (c, d) of KeTo. Cells were grown in enriched liquid EMJH for 5 days at 30 °C under darkness at shake cultures (120 rpm). Asterisks and arrows represent aggregates and single cells of KeTo, respectively. Scale bar in (a) and (b), 100 µm. Scale bar in (c) and (d), 500 nm and 200 nm, respectively.