Fig. 6

Activation marker expressions in DCs and macrophages at the site of injection after vaccination. Mice were vaccinated with sPR8 vaccine alone or adjuvanted (PC, MPL). Muscles (a, b) and ILN (c, d) samples were harvested at 3 days post-boost vaccination and the activation marker expressions on DCs and macrophages were acquired by flow cytometry. DCs and macrophages were gated by CD11c and CD11b surface markers, respectively. Means ± standard errors are shown with individual dots. One-way ANOVA and Tukey’s multiple comparison test were performed for statistical analysis. *p < 0.0332; **p < 0.0021; ***p < 0.0002; ****p < 0.0001 compared to between the indicated groups.