Fig. 3
From: Salbutamol attenuates arrhythmogenic effect of aminophylline in a hPSC-derived cardiac model
Synergic effect of salbutamol and aminophylline on contractile properties of hPSC-CMs. *stands for P ≤ 0.05, **P ≤ 0.01, compared to control, # stands for P ≤ 0.05 and #### stands for P ≤ 0.0001. Scatter plots with indicated means and standard deviation (SD) of the beat rate (A) and contraction force (B) relative responses normalized to the respective baseline control measurement in the case of salbutamol and aminophylline and to mean values of the control measurement in the case of control (n = 17 for controls, n = 13, 13, 11, 10, 10, 15, 15, 23, and 15 for treatments, P values in Table S1 in Supplementary data, Brown-Forsythe and Welch ANOVA test was used for contraction force results, ordinary one-way ANOVA for the beat rate results and Kruskal-Wallis test was applied to compare contraction force and beat rate results of mixes with individual treatment). The legend in A applies to the whole graph. The results of the mixes consist of two separate experiments, same control dataset used based on time of measurement (the first two controls are the same as the third and fourth, relating to 20 min and 40 min of treatment). The results of separate aminophylline administration originate from our previous paper6. (A, B) The beat rate and contraction force significantly differed from controls in mixed treatments with 1 mM aminophylline and 10 nM, 100 nM, or 1 µM salbutamol. Still, no significant effect was observed in individual treatments. The effect of mixed treatments was amplified when compared to individual treatments.
