Figure 1 | Scientific Reports

Figure 1

From: Prader-Willi syndrome protein necdin regulates the nucleocytoplasmic distribution and dopaminergic neuron development

Figure 1

Necdin interacts with THOC5. (a) Interaction of necdin and THOC5 as assayed with a yeast two-hybrid assay. pGBKT7-Ndn denotes necdin fused to a DNA-binding domain, whereas pGADT7-Thoc5 denotes THOC5 fused to an activation domain. Only yeast colonies co-transformed with pGADT7-Thoc5 and pGBKT7-Ndn can grow and turn blue on an SD/-Leu/-Trp/AbA/X-α-Gal plate. (b) Interaction of necdin and THOC5 as assayed by a co-immunoprecipitation (co-IP) assay in HEK293T cells expressing FLAG-necdin and MYC-THOC5. Antibodies against MYC or FLAG were used to immunoprecipitate (IP) the cell lysate, and the immunocomplex was blotted with antibodies against MYC or FLAG, respectively. (c) Interaction of necdin and THOC5 as assayed by a co-IP assay in SH-SY5Y cells. The cell lysate was immunoprecipitated with an antibody against necdin or control IgG, and the immune complex was blotted with antibodies against necdin or THOC5, respectively. Equal amounts of protein from whole cell lysates were used in immunoblotting for the indicated proteins.

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