Table 3 Quantitative reverse transcription polymerase chain reaction (RT-qPCR) values in runners and sedentary animals.

From: Running in mice increases the expression of brain hemoglobin-related genes interacting with the GH/IGF-1 system

Transcript

Group

Prefrontal Cortex

Hippocampus

Motor Cortex

Aggregated#

  

Mean (95% CI)

Mean (95% CI)

Mean (95% CI)

Mean (95% CI)

Hbb

Runner

146.2 (97.1–195.3)

163.7 (109.3–218.0)

161.9 (114.3–209.5)

157.2 (117.3–197.2)

 

Sedentary

100.0 (71.1–128.9)

100.0 (69.8–130.2)

100.0 (71.1–128.9)

100.0 (60.0–140.0)

Alas2

Runner

147.2 (100.8–193.6)

159.4 (110.4–208.3)

150.6 (107.4–193.7)

152.4 (116.0–188.8)

 

Sedentary

100.0 (72.0–128.0)

100.0 (73.7–126.3)

100.0 (73.0–127.0)

100.0 (63.6–136.4)

Alox15

Runner

76.6 (63.9–89.4)

125.9 (81.8–170.1)

163.5 (117.6–209.5)

123.0 (95.8–150.2)

 

Sedentary

100.0 (66.3–133.7)

100.0 (60.3–139.7)

100.0 (43.3–156.7)

99.7 (72.6–126.8)£

BDNF

Runner

105.5 (84.5–126.5)

99.5 (90.4–108.6)

91.3 (78.1–104.5)

98.8 (87.9–109.7)

 

Sedentary

100.0 (89.0–111.0)

100.0 (89.6–110.4)

100.0 (88.7–111.3)

100.0 (89.1–110.9)

Bax

Runner

94.5 (91.6–97.4)

96.8 (89.9–103.7)

97.8 (94.1–101.6)

96.4 (93.3–99.5)

 

Sedentary

100.0 (95.0–105.0)

100.0 (94.5–105.5)

100.0 (96.4–103.6)

100.0 (96.9–103.1)

Grin2a

Runner

91.9 (85.1–98.7)

100.4 (96.1–104.7)

92.6 (83.5–101.7)

95.0 (91.0–98.9)

 

Sedentary

100.0 (91.1–108.9)

100.0 (95.4–104.6)

100.0 (93.5–106.5)

100.0 (96.1–103.9)

Grin2b

Runner

90.3 (85.9–94.8)

99.2 (90.4–107.9)

94.3 (87.6–100.9)

94.6 (90.9–98.3)

 

Sedentary

100.0 (93.6–106.4)

100.0 (96.0–104.0)

100.0 (94.9–105.1)

100.0 (96.3–103.7)

Hif1a

Runner

100.5 (94.2–106.8)

103.5 (98.7–108.3)

106.4 (101.6–111.3)

103.5 (99.4–107.5)

 

Sedentary

100.0 (94.4–105.6)

100.0 (94.8–105.2)

100.0 (95.5–104.5)

100.0 (96.0–104.0)

Gfap

Runner

104.8 (93.5–116.2)

95.3 (89.8–100.9)

93.2 (86.1–100.2)

100.8 (93.2–108.5)

 

Sedentary

100.0 (85.5–114.5)

100.0 (91.0–109.1)

100.0 (85.5–114.5)

100.0 (92.4–107.6)

Igf1

Runner

96.5 (84.4–108.6)

98.8 (93.5–104.2)

95.7 (89.9–101.5)

97.0 (92.1–101.9)

 

Sedentary

100.0 (94.1–105.9)

100.0 (95.6–104.4)

100.0 (94.4–105.6)

100.0 (95.1–104.9)

Igf1r

Runner

85.2 (80.9–89.6)

90.9 (86.9–94.9)

88.1 (81.9–94.2)

88.1 (84.8–91.3)

 

Sedentary

100.0 (95.5–104.5)

100.0 (95.1–104.9)

100.0 (95.3–104.7)

100.0 (96.8–103.2)

Insr

Runner

86.8 (81.8–91.7)

88.7 (82.8–94.7)

87.3 (83.7–91.0)

87.6 (83.4–91.8)

 

Sedentary

100.0 (94.9–105.1)

100.0 (93.1–106.9)

100.0 (94.9–105.1)

100.0 (95.8–104.2)

Ghr

Runner

90.2 (81.6–98.8)

102.8 (92.5–113.1)

113.8 (100.8–126.8)

102.3 (95.1–109.4)

 

Sedentary

100.0 (92.9–107.1)

100.0 (92.8–107.2)

100.0 (90.1–109.9)

100.0 (92.9–107.1)

  1. The values are normalized against sedentary = 100. Mean and variation is given as 95% confidence intervals (95% CI). Significant differences (p < 0.05) are in bold and designate t-test comparisons between runners and sedentary for each brain region. #For all three brain regions aggregated, the mixed model was used for statistical comparison between sedentary and runner (see Methods). The average sedentary values for each brain region were normalized to 100. £The reason for that sedentary was 99.7 for Alox15 in the three brain regions were some missing values (motor cortex; n = 2, prefrontal cortex; n = 1, hippocampus; n = 1). The mixed model algorithm considers this and balances missing values slightly, which slightly changed the calculated mean for this group. The other transcripts had no missing values, and adjusted sedentary means were all 100.0. There were however also missing values in the running group (prefrontal cortex; n = 3, hippocampus; n = 1), which also slightly changed the calculated means (but this is not as easily observed, as means were not expected to be 100). This is also graphically shown in Fig. 2. Transcript abbreviations are found in Table 1.
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