Fig. 2 | Scientific Reports

Fig. 2

From: Molecular mechanisms at the basis of the protective effect exerted by EPPS on neurodegeneration induced by prefibrillar amyloid oligomers

Fig. 2

(A) Dose-Response curve (MTS assessment) obtained after 1 h of EPPS treatment, in differentiated HT22 cells. In the concentration range between 0.5mM and 5mM, cell viability is not affected and it reachs the half-maximal Inhibitory Concentration (IC50) at 241 ± 34 mM. (B) Biological effects induced on differentiated HT22 cells by 24 h of EPPS different treatments evaluated by the MTS assessment: in green, with sCT-NA (8µM) solution (n = 15 wells from N = 4 experiments); in red to test the PFOs toxicity, with sCT-PFOs (8µM) (n = 22 wells from N = 6 experiments) (**p < 0.01 with respect to CTRL in black, ); in brown to validate the Dose-Response curve obtained after only 1 h of EPPS treatment, with EPPS (3mM)(n = 3 wells from N = 2 experiments); in blue to test the EPPS protection effect, with EPPS (3mM) + PFOs (8µM) (n = 17 wells from N = 5 experiments). (C) The same biological effects, evaluated by LDH assessment (in the ordinate axis the Relative Luminiscence Unit % is indicated).

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