Fig. 4

Relative gene-expression of neurexin-1 (Nxn1) and neuroligin-1 (Nlgn1) (functional synaptic markers) in the lesioned spinal cord tissue. A, C, E: relative change of Nxn1 mRNA; B, D, F: relative change of Nlgn1 mRNA. Spinal cord section analyzed (relative to T10): A, B: cephalic (rostral) – T9-T8; C, D: injury site (epicenter)—T10-T11; E, F: caudal (distal)—T12-T13. Experimental groups: sham (control), spinal cord injury (SCI), GnRH, GH and GnRH + GH (G + G). RPS18: reference gene. Values are represented as mean ± SEM. At least 5 animals are included per group from 2 independent experiments. One-way Brown-Forsythe and Welch ANOVA with Fisher´s LSD post-hoc test identified significant differences. A p-value under 0.05 indicated statistical significance and is represented with asterisks as follow: * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.