Fig. 3
DNA methylation of the PRKCB promoter in gastric cancer. (a) Schematic diagram showing the filtering scheme for DMGs in this study. (b) DNA methylation level and the average change of PRKCB, IKZF1, FLT3, SMPD5 and KCNN2 in gastric cancer tissues and adjacent tissues. (c) The scatter plot shows the average PRKCB gene methylation (vertical axis) of different samples (dots) in three prominent DMPs (horizontal axis). (d) DNA methylation levels of PRKCB-cg08406370, PRKCB-cg00735962, and PRKCB-cg18526361 by pyrosequencing. P-values are shown between the indicated groups, determined by a two-sided paired Student’s t-test. *P < 0.05. (e) DNA methylation levels of PRKCB-cg08406370 (P = 0.00096) and PRKCB-cg00735962 (P = 0.00024) was higher in gastric cancer tissues compared with adjacent normal tissue. (f) ROC curve of the Δβ methylation values for PRKCB-cg08406370 and PRKCB-cg00735962 in gastric cancer patients. AUC of the ROC curves: 0.81 for PRKCB-cg00735962, 0.76 for PRKCB-cg08406370. AUC, area under the curve; ROC, receiver operating characteristic.
