Fig. 4
LINC01320 recruits PURB to the DDB2 promoter. (A) Diagram showing the PNRs in the promoter region of DDB2. (B, C) ChIP-qPCR of PURB-associated DNA sequences in the PURB-binding region of the DDB2 promoter in (B) SKOV3 and (C) OVCAR3 cells. The GAPDH gene was used as a negative control, n = 3 per group. (D) After treatment of OC cells with OE-EV, OE-PURB, or OE-PURB + si-LINC01320 for 48 h, DDB2 expression levels were determined by qRT-PCR assays, n = 4 per group. (E) The dual luciferase reporter assay measured the luciferase activity of wild-type (WT) and mutant (Mut) DDB2 reporter genes, n = 6 per group. (F, G) After treatment of OC cells with OE-EV, OE-PURB, or OE-PURB + si-LINC01320 for 48 h, dual luciferase report analysis verified the luciferase activity of WT and Mut DDB2 reporter genes in SKOV3 (F) and OVCAR3 (G) cells, n = 6 per group. (H, I) After treatment of OC cells with si-NC or si-LINC01320 for 48 h, ChIP-qPCR of PURB-associated DNA sequences in the PURB binding region of the DDB2 promoter in SKOV3 (H) and OVCAR3 (I) cell lines transfected with si-LINC01320 or si-NC, n = 3 per group. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant.
