Fig. 3
From: Rac1 inhibition regenerates wounds in mouse fetuses via altered actin dynamics
Effect of NSC23766 on wound healing in mouse fetuses. (a) Macroscopic analysis of the scar 72 h after the administration of NSC23766 to embryonic day (E)14. The upper panel shows the analysis of 2D photographs and the lower panel shows the analysis of 3D images. Yellow dotted line: area of visible mark. Scale bar = 1 mm. (b) Quantitative comparison of wound depth and area 72 h after injury following NSC23766 administration at E14. *P < 0.05. (c) Hematoxylin & eosin-stained images of the scar 72 h after the administration of NSC23766 to embryonic day (E)14. Skin structures completely regenerated in the NSC23766-treated group. Black arrows: area of the wound; yellow triangles: tips of the panniculus carnosus muscle. Scale bar = 200 μm. (d) Regeneration of panniculus carnosus muscle 72 h after the administration of NSC23766 to embryonic day (E)14 (fluorescent staining of desmin). Red: desmin; blue: DAPI (nuclei); white arrows: area of the wound; yellow triangles: tips of the panniculus carnosus muscle. Scale bar = 200 μm. (e) Immunostaining for actin in the wound epidermis at E14 and 36 h after NSC23766 administration. Green: actin; blue: DAPI (nuclei); yellow dotted line: wound margins. Scale bar = 20 μm. (f) Evaluation of Rac1 activity in the wound epidermis at E14 at 36 h after NSC23766 administration and wounding. *P < 0.05.
