Fig. 2

Generation and purification of DNA nanoparticles from GFP gene-containing scaffolds. Representative agarose gel electrophoresis display of the purified DNA nanoparticles used in this study. M: molecular marker; 1: dsGFP (fully duplex GFP gene); 2: ssGFP (single-stranded antisense strand (i.e., scaffolds for DNA nanoparticles)); GHL: DNA nanoparticles with linear promoter/enhancer; 3: PO (GHL PO; DNA nanoparticle with linear promoter/enhancer and single-stranded gene); 4: HS (GHL HS; DNA nanoparticle with linear promoter/enhancer and a half complement of staples); 5: FS (GHL FS; DNA nanoparticle with linear promoter/enhancer and a mostly full complement of staples); 6: FSC (GHL FSC; DNA nanoparticle with linear promoter/enhancer and a full complement of staples). For lanes 1 and 2, the band at approximately 1.7 kb represents dsGFP, and the band at approximately 0.7 kb represents ssGFP. The bands between 1.7 kb and 1 kb for lanes 3, 4, 5, and 6 represent successfully self-assembled DNA nanoparticles with linear duplex promoter/enhancer.