Fig. 5 | Scientific Reports

Fig. 5

From: Hypoxic postconditioning modulates neuroprotective glial reactivity in a 3D cortical ischemic-hypoxic injury model

Fig. 5

Dynamics of Neuronal and Astrocytic Spreading Post-Hypoxic Postconditioning. (a) Schematic sequence of the 2D spreading assay post-OGD-R and various HPC treatments. 3D cortical spheroids were placed on PDL-coated glass slides on DIV 13 to monitor neurite and astrocyte process extension over three days. (b) Sequential images capturing the spreading of control, OGD-R, and HPC-treated spheroids across time points. (c) Time-series plot of the normalized spreading area with statistical significance markers against OGD-R spheroids and control. Data are represented as mean ± SD (n = 8–10 spheroids per condition) (*p < 0.05). (d) Immunofluorescence staining displays β-III-tubulin (red) for neuronal processes and GFAP (green) for astrocytic processes in each group with enlarged view. Scale bars denote 200 μm.

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