Fig. 6 | Scientific Reports

Fig. 6

From: Hypoxic postconditioning modulates neuroprotective glial reactivity in a 3D cortical ischemic-hypoxic injury model

Fig. 6

HPC-Induced Alterations in Astrocyte Reactivity and Proliferation in a Cortical Ischemic-hypoxic injury model. (a) Immunofluorescence images depicting GFAP expression (green) and nuclei (DAPI, blue) in control and treated spheroids with enlarged view of GFAP. Scale bars denote 200 μm and 50 μm for normal and enlarged view respectively. (b) Immunofluorescence for Ki67 (green) indicates proliferative activity against a backdrop of nuclei (DAPI, blue). (c) Quantitative analysis of GFAP fluorescence intensity. (d) Analysis of Ki67 fluorescence intensity. (e) The bar graph shows the relative mRNA expression levels of astrocytic markers s100a10 and C3 across all groups. Scale bars represent 200 μm. Data are expressed as means ± standard deviation (SD) (n = 5, 8–10 spheroids per condition) (*p < 0.05 indicates statistical significance).

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