Fig. 4

Far UV circular dichroism (CD) spectra of 0.05 mg/mL doubly His-tagged UB (top row A, B) or Cas9 (bottom row C, D) with 10 µM NiCl2 (left hand panels - -) or ZnCl2 (right hand panels - -) in DDW at 25 °C. Spectra measured in the absence of metal ions (---) were used as control. Background has been subtracted.