Fig. 2
Resveratrol protects INS1E-hIAPP cells from an altered energetic homeostasis through changes in mitochondrial dynamics, mTORC1 and ER-stress signalling pathways. (A) Immunoblot analysis of Mfn1, Mfn2, p-Drp1, Drp1, OPA1, HADHA, using β actin as loading control, in the cell extracts of both INS1E-WT and INS1E-hIAPP pancreatic β cell lines exposed to either low or high glucose levels with and without Resveratrol 30 µM 4 h (n = 5). The plot indicates the quantification data of Mfn1, Mfn2, p-Drp1/Drp1, L-OPA1/S-OPA1 HADHA/β-actin ratio under the different conditions. Data represent the mean ± standard error of the mean (SEM). *p < 0.05; **p < 0.01 using ANOVA test using as post hoc the Tukey’s multiple comparison test. (B) Immunoblot analysis of p-p70, p70, p-ULK1 757, ULK1 using β actin as loading control, in the cell extracts of both INS1E-WT and INS1E-hIAPP pancreatic β cell lines exposed to either low or high glucose levels with and without Resveratrol 30 µM 4 h (n = 5). The plot indicates the quantification data of Mfn1, Mfn2, p-p70/p70, p-ULK1 757/ULK1 ratio under the different conditions. Data represent the mean ± standard error of the mean (SEM). *p < 0.05 by unpaired Student’s t-test (C) Immunoblot analysis of BIP, p-eIF2α, eIF2α, p-PERK, PERK, using β actin as loading control, in the cell extracts of both INS1E-WT and INS1E-hIAPP pancreatic β cell lines exposed to either low or high glucose levels with and without Resveratrol 30 µM 4 h (n = 5). The plot indicates the quantification data of BIP, p-eIF2α/eIF2α, p-PERK/PERK ratio under the different conditions. Data represent the mean ± standard error of the mean (SEM). *p < 0.05; **p < 0.01 by unpaired Student’s t-test.
