Fig. 5
Hippocampal cells HT-22 are protected from the aggregate-prone hIAPP medium with resveratrol. (A) Immunoblot analysis of amylin, using β actin as loading control, in the HT-22 cell lines exposed to conditioned media for 0, 8 and 24 h with and without Resveratrol 30 µM 4 h (n = 5). The plot indicates the quantification data of Amylin/β-actin ratio under the different conditions. Data represent the mean ± standard error of the mean (SEM) (n = 5). (B) Immunofluorescence staining using an antibody against amylin and MG5 compound in the HT-22 cells exposed to conditioned media for 24 h with and without Resveratrol 30 µM 4 h. DAPI staining was used to detect nuclei. The plot indicates the quantification data of both, amylin fluorescence intensity and MG5 fluorescence intensity under the different conditions. **p < 0.01; ****p < 0.0005 by unpaired Student’s t-test.
